SCREENING OF RUTIN FROM LEAVES EXTRACTS OF WILD PLANTS IN PALESTINE

Mona A. Ahmad, Iqab Ghaleb Naji

Abstract


Rutin is a source of flavonoids that have antioxidant activity also reduce the risk of cardio vascular disease and provide cardio protective results. Rutin show anticancer action, anti-inflammatory action and vitamin C boost. The aim of this study was to screen the Rutin in leaves extracts of Salvia Judaica Boiss, Rumex acetosa, Arum palaestinum, Portulaca oleracea and Teucrium polium that grow in Palestine as wild plants usinf HPLC. The HPLC separation was performed using a C18 sunfire column (3.5mm 4.6 x 150 mm). The mobile phase consisted of phosphate buffered saline (pH = 3.5) and acetonitrile (30/70, v/v). The flow rate was set at 1.00 ml/min and the effluent was monitored at 356 nm. Rutin was eluted at 1.1 min and the peak area was compared to standard. Excellent linear correlation coefficient was observed in the range of 0.985 to 0.987. Excellent linear correlation coefficient was observed in the range of 0.985 to 0.987. The reproducibility of the method was 1.49 % (RSD) and accuracy was confirmed with average recovery ranging from 95.62 to 98.12 %.


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ISSN: 2332-2160

Impact Factor = 0.465 (2013)